Flow Cytometry at Aberystwyth
What is Flow Cytometry?
Cytometry refers to the measurement of the physical and chemical
characteristics of cells. By extension, flow cytometry refers to the technique
where such measurements are made as the cells pass in a fluid stream through a
measuring point surrounded by an array of detectors.
The following flow cytometry web pages are available here at
How to use the Skatron flow
How to use the Coulter Epics Elite
How to prepare your samples for our
Aberystwyth flow cytometry-related
(a beginner's guide to flow cytometry)
Flow Cytometry -
Other flow cytometry sites
If you have Netscape 3 / Microsoft Internet
Explorer 3 or later you can view the following powerpoint
A portable flow cytometer for
the detection and identification of microorganisms
Application of flow cytometry
in biowarfare agent detection: A portable diode-based instrument and a
Still want to know more? For more information on the flow
cytometry of microorganisms see the following reviews:
- Alberghina L, and Porro D: Quantitative flow cytometry:
analysis of protein distributions in budding yeast. - a mini review. Yeast.
- Amann RI, Ludwig W, and Schleifer KH: Phylogenetic
identification and in situ detection of individual microbial cells without
cultivation. Microbiological Reviews. 59:143-169, 1995.
- Camplejohn RS: The measurement of intracellular antigens and
DNA by multiparametric flow cytometry. Journal of Microscopy. 176:1-7, 1994.
- Cunningham A, and Leftey JW: Application of flow cytometry to
algal physiology and phytoplankton ecology. FEMS Microbiology Reviews.
- Davey HM, and Kell DB: Flow cytometry and cell sorting of
heterogeneous microbial populations: the importance of single-cell analyses.
Microbiological Reviews. 60:641-696, 1996.
- Fouchet P, Jayat C, Héchard Y, Ratinaud M-H, and Frelat
G: Recent advances of flow cytometry in fundamental and applied
microbiology. Biology of the Cell. 78:95-109, 1993.
- Frelat G, Laplace-Builhe C, and Grunwald D: Microbial analysis
by flow cytometry: Present and future, In Flow cytometry: Advanced research and
clinical applications (volume II), Yen A (ed.) CRC Press, Boca Raton FL. 1989,
- Kaprelyants AS, Gottschal JC, and Kell DB: Dormancy in
non-sporulating bacteria. FEMS Microbiology Reviews. 104:271-286, 1993.
- Pore RS: Antibiotic susceptibility testing by flow cytometry.
Journal of Antimicrobial Chemotherapy. 34:613-627, 1994.
- Shapiro HM: Flow cytometry in laboratory microbiology: New
directions. ASM News. 56:584-588, 1990.
- Troussellier M, Courties C, and Vaquer A: Recent applications
of flow cytometry in aquatic microbial ecology. Biol. Cell. 78:111-121, 1993.
- Vesey G, Narai J, Ashbolt N, Williams K, and Veal D: Detection
of specific microorganisms in environmental samples using flow cytometry.
Methods In Cell Biology. 42:489-522, 1994.
There are many other flow cytometry resources on the world wide
web, here is a list of some of
For a summary of the information held on this web server and many more
links go to the index.
Author: Hazel Davey