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[FLOW WEB]

Flow Cytometry at Aberystwyth


What is Flow Cytometry?

Cytometry refers to the measurement of the physical and chemical characteristics of cells. By extension, flow cytometry refers to the technique where such measurements are made as the cells pass in a fluid stream through a measuring point surrounded by an array of detectors.

The following flow cytometry web pages are available here at Aberystwyth...

Flow cytometers

Flow cytometrists

How to use the Skatron flow cytometer

How to use the Coulter Epics Elite flow cytometer

How to prepare your samples for our flow cytometers

Aberystwyth flow cytometry-related publications

Software

Microcyte (a beginner's guide to flow cytometry)

Flow Cytometry - Cymraeg

Other flow cytometry sites

If you have Netscape 3 / Microsoft Internet Explorer 3 or later you can view the following powerpoint presentations:

A portable flow cytometer for the detection and identification of microorganisms

Application of flow cytometry in biowarfare agent detection: A portable diode-based instrument and a multiparameter approach.


Still want to know more?

For more information on the flow cytometry of microorganisms see the following reviews:
  1. Alberghina L, and Porro D: Quantitative flow cytometry: analysis of protein distributions in budding yeast. - a mini review. Yeast. 9:815-823, 1993.
  2. Amann RI, Ludwig W, and Schleifer KH: Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews. 59:143-169, 1995.
  3. Camplejohn RS: The measurement of intracellular antigens and DNA by multiparametric flow cytometry. Journal of Microscopy. 176:1-7, 1994.
  4. Cunningham A, and Leftey JW: Application of flow cytometry to algal physiology and phytoplankton ecology. FEMS Microbiology Reviews. 32:159-164, 1986.
  5. Davey HM, and Kell DB: Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiological Reviews. 60:641-696, 1996.
  6. Fouchet P, Jayat C, Héchard Y, Ratinaud M-H, and Frelat G: Recent advances of flow cytometry in fundamental and applied microbiology. Biology of the Cell. 78:95-109, 1993.
  7. Frelat G, Laplace-Builhe C, and Grunwald D: Microbial analysis by flow cytometry: Present and future, In Flow cytometry: Advanced research and clinical applications (volume II), Yen A (ed.) CRC Press, Boca Raton FL. 1989, pp. 256-279.
  8. Kaprelyants AS, Gottschal JC, and Kell DB: Dormancy in non-sporulating bacteria. FEMS Microbiology Reviews. 104:271-286, 1993.
  9. Pore RS: Antibiotic susceptibility testing by flow cytometry. Journal of Antimicrobial Chemotherapy. 34:613-627, 1994.
  10. Shapiro HM: Flow cytometry in laboratory microbiology: New directions. ASM News. 56:584-588, 1990.
  11. Troussellier M, Courties C, and Vaquer A: Recent applications of flow cytometry in aquatic microbial ecology. Biol. Cell. 78:111-121, 1993.
  12. Vesey G, Narai J, Ashbolt N, Williams K, and Veal D: Detection of specific microorganisms in environmental samples using flow cytometry. Methods In Cell Biology. 42:489-522, 1994.

There are many other flow cytometry resources on the world wide web, here is a list of some of them.

For a summary of the information held on this web server and many more links go to the index.


Author: Hazel Davey hlr@aber.ac.uk

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