Some issues to consider when developing Coulter Epics Elite protocols

• What excitation wavelength(s) do you need? We have the following lasers available:
  • Helium Cadmium (325 nm)
  • Argon Ion (488 nm)
  • Green Helium Neon (544 nm)
  • Red Helium Neon (633 nm)
• What emission wavelength(s) do you need? We have the following filters available:
• Is the staining time dependent?
• Use the Opticad screen to record your filter setup for future reference
• Remember to save your protocol!!